25 - Hydroxy Vitamin D
The storage form of vitamin D. Deficiency is near-universal in Indian cities and sits behind bone pain, tiredness and low mood
Also known as Vit D, 25-OH vitamin D, Bone health test, Calciferol, Vitamin D (25-Hydroxy), Vitamin D
₹820/-₹1,400/-41% offFree collection on orders above ₹500/-
- Reports within
- 8 hours
- Parameter included
- 01see list
- Sample
- Blood
- Fasting
- Not needed
- Measures
- Level of 25-hydroxy vitamin D in the blood
- Identifies
- Vitamin D deficiency or insufficiency, and whether supplements are working
Collected at home
A trained phlebotomist visits at a time you pick, from the next hour
Sealed single-use kit
Opened in front of you, and carried to the lab under controlled conditions
Report within 8 hours
On WhatsApp and email, with any value outside the normal range highlighted
What is the Vitamin D test?
Vitamin D is made in the skin from sunlight and helps the body absorb calcium for bones, muscles and immunity. The test measures 25-hydroxy vitamin D, the storage form that circulates in the blood, in nanograms per millilitre. It is the best single indicator of how much vitamin D you actually have.
A low result is the usual explanation for aching bones, low back pain, muscle weakness, tiredness and low mood in people who otherwise look well. Deficiency is extremely common in Indian cities, where most of the day is spent indoors and sunscreen, clothing and pollution block what sun there is. The number tells your doctor whether you need a short high-dose course, a maintenance dose, or nothing at all, and a repeat after three months shows whether the dose worked.
No fasting is needed. The sample is collected at home at any time of day and the report arrives in 8 hours.
Vitamin D parameters1 measured
Vitamin D (25-OH)
30–100 ng/mL
The storage form of vitamin D made from sunlight and diet, low with indoor living, darker skin or covered clothing and linked to weak bones and fatigue
Reference ranges are the lab's defaults for adults and are printed on the report beside every value
How to prepare
- No fasting is needed.
- Tell the phlebotomist if you have taken a high-dose vitamin D sachet or injection in the past few weeks, as it raises the result.
- Take your regular medicines as usual.
Who should take it
Anyone with bone pain, back pain, muscle aches, cramps or unexplained tiredness. People who spend most of the day indoors, work night shifts, or cover their skin outdoors. Adults over 50, post-menopausal women and anyone with osteoporosis or a low-impact fracture. People with dark skin, obesity, kidney or liver disease, or gut conditions such as coeliac disease that reduce absorption. Pregnant and breastfeeding women. Anyone taking vitamin D supplements who wants to know whether the dose is enough, or too much.
Before the visit
Tell your doctor about any vitamin D, calcium or multivitamin supplements, and about steroids, anti-epileptic drugs or weight-loss medicines, which can lower the level. Very high results from over-supplementation can raise blood calcium and need attention.
Why take the Vitamin D test?
- Bone or back pain: aching bones, hip or lower back pain and muscle weakness with no injury are the commonest signs of low vitamin D.
- Tiredness and low mood: persistent fatigue, poor sleep and low mood often improve once a deficiency is corrected.
- Little sun: office workers, night-shift staff and anyone who covers up outdoors make almost none of their own vitamin D.
- Bone health check: post-menopausal women, people with osteoporosis or a fracture from a small fall, and anyone on long-term steroids.
- Monitoring supplements: repeating the test after a course of vitamin D shows whether the level has reached the target range.
Questions about the Vitamin D test
What is a vitamin D test used for?
It measures your vitamin D stores to explain bone or back pain, muscle aches, tiredness and low mood, and to guide how much supplement you need. It is repeated after a course to check the level has come up.
Do I need to fast for a vitamin D test?
No fasting is needed. Eat normally and take your usual medicines. Tell the phlebotomist if you have had a high-dose vitamin D sachet or injection recently, as it raises the reading.
What sample is taken and how does home collection work?
A small blood sample from the arm. A trained phlebotomist visits your home with a sealed single-use kit and takes the sample to the lab. Any time of day is fine.
What is the normal vitamin D range?
The report gives 25-hydroxy vitamin D with a reference range of 30 to 100 ng/mL. Below 30 is insufficient, and well below that is deficient. Above 100 suggests too much supplementation.
What does a low vitamin D result mean?
It means your stores are short, usually from too little sun and too little in the diet. Your doctor will decide between a short high-dose course and a daily maintenance dose, and often adds calcium. Very low levels in children can affect bone growth, so they are treated promptly.
Who should get vitamin D tested and how often?
Anyone with bone pain, muscle aches or unexplained tiredness, people who spend the day indoors, adults over 50, post-menopausal women, and pregnant women. Test once, then recheck three months after starting supplements.
How long does the vitamin D report take?
The report reaches your WhatsApp and email in 8 hours from collection. Keep it, because the next reading after a course of supplements is compared against this one.
I get plenty of sun. Can I still be low in vitamin D?
Yes, and most people tested in Indian cities are. Skin makes vitamin D only from midday sun on bare skin, and clothing, sunscreen, glass, pollution and darker skin all cut it down. Being outdoors in the morning or evening helps less than people assume.
Commonly tested together
Often on the same prescription
What happens after you book
- A phlebotomist is assigned and reaches you in your chosen slot, as soon as the next hour
- The sample is collected with a sealed single-use kit and moved on a cold chain
- The report lands on WhatsApp and email within 8 hours